Systematic profiling of cellular phenotypes with s...[Genome Biol. 2006]

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1: Genome Biol. 2006;7(1):R6. Epub 2006 Jan 31. Links

Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes.

Narayanaswamy R, Niu W, Scouras AD, Hart GT, Davies J, Ellington AD, Iyer VR, Marcotte EM.

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA.

We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.

PMID: 16507139 [PubMed - indexed for MEDLINE]

PMCID: PMC1431703

Combining mutations in the incoming and outgoing pheromone signal pathways causes a synergistic mating defect in Saccharomyces cerevisiae.
Yeast. 1999 Jun 30; 15(9):765-80.

[Yeast. 1999]
Identification and characterization of a Candida albicans mating pheromone.
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[Mol Cell Biol. 2003]
Global roles of Ste11p, cell type, and pheromone in the control of gene expression during early sexual differentiation in fission yeast.
Proc Natl Acad Sci U S A. 2006 Oct 17; 103(42):15517-22. Epub 2006 Oct 9.

[Proc Natl Acad Sci U S A. 2006]
ReviewIdentification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection.
Methods Enzymol. 2004; 389:399-409.

[Methods Enzymol. 2004]
ReviewGlobal synthetic-lethality analysis and yeast functional profiling.
Trends Genet. 2006 Jan; 22(1):56-63. Epub 2005 Nov 23.

[Trends Genet. 2006]
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Cited by 5 PubMed Central articles

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[Proc Natl Acad Sci U S A. 2009]
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Zhao J, Niu W, Yao J, Mohr S, Marcotte EM, Lambowitz AM. PLoS Biol. 2008 Jun 24; 6(6):e150.

[PLoS Biol. 2008]
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