A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate-polyacrylamide electrophoresis gels

Proteomics. 2006 Apr;6(8):2334-7. doi: 10.1002/pmic.200500596.

Abstract

A quick, sensitive, and MALDI-TOF MS compatible silver staining method, namely Eriochrome black T (EBT)-silver method, is described. The method can detect 0.05-0.2 ng protein within 60 min in SDS-PAGE gels. EBT dye was used as a silver ion sensitizer having reducing power for silver ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Electrophoresis, Polyacrylamide Gel / instrumentation*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Mass Spectrometry / methods*
  • Proteomics / instrumentation*
  • Proteomics / methods*
  • Silver Staining / methods*

Substances

  • Azo Compounds
  • Eriochrome Black T