Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica

Milan Jirkù; Eva Zemanová; Amer Al-Jawabreh; Gabriele Schönian; Julius Lukes

doi:10.1016/j.diagmicrobio.2005.12.001

Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica

Diagn Microbiol Infect Dis. 2006 May;55(1):75-9. doi: 10.1016/j.diagmicrobio.2005.12.001. Epub 2006 Feb 20.

Authors

Milan Jirkù¹, Eva Zemanová, Amer Al-Jawabreh, Gabriele Schönian, Julius Lukes

Affiliation

¹ Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice, Czech Republic.

PMID: 16490336
DOI: 10.1016/j.diagmicrobio.2005.12.001

Abstract

We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Animals
Child
Child, Preschool
Cricetinae
DNA, Protozoan / analysis
Diagnosis, Differential
Humans
Leishmania tropica / genetics*
Leishmania tropica / isolation & purification
Leishmaniasis / diagnosis*
Leishmaniasis / parasitology
Male
Mesocricetus
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity
Species Specificity

Substances

DNA, Protozoan