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J Am Chem Soc. 2006 Feb 22;128(7):2214-5.

Kinase-catalyzed modification of gold nanoparticles: a new approach to colorimetric kinase activity screening.

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  • 1Centre for Nanoscale Science, Department of Chemistry and School of Biological Sciences, The University of Liverpool, Liverpool L69 7ZD, U.K.


Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity.

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