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Methods Enzymol. 2005;403:243-53.

Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosis.


The endocytic and trafficking route followed by proteins that enter cells independently of clathrin is not well understood. In HeLa cells, a distinct endocytic and recycling pathway is followed for such clathrin-independent cargo proteins, and we have characterized the role of Rab proteins in this process. Here we describe cell-based flourescence assays to examine the effects of expression and depletion of Rab22 on endosomal morphology and endocytic recycling.

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