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J Bacteriol. 1991 Jun;173(12):3914-7.

Bacterial DNA supercoiling and [ATP]/[ADP] ratio: changes associated with salt shock.

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  • 1Public Health Research Institute, New York, New York 10016.

Abstract

When Escherichia coli K-12 was shifted from a medium lacking salt to one containing 0.5 M NaCl, both the [ATP]/[ADP] ratio and negative supercoiling of plasmid DNA increased within a few minutes. After about 10 min both declined, eventually reaching a level slightly above that observed with cells growing exponentially in the absence of salt. Since in vitro the [ATP]/[ADP] ratio influences the level of supercoiling generated by gyrase (H. Westerhoff, M. O'Dea, A. Maxwell, and M. Gellert, Cell Biophys. 12:157-181, 1988), the physiological response of supercoiling to salt shock is most easily explained by the sensitivity of gyrase to changes in the intracellular [ATP]/[ADP] ratio. This raises the possibility that the [ATP]/[ADP] ratio is an important factor in the control of supercoiling.

PMID:
1646791
PMCID:
PMC208027
[PubMed - indexed for MEDLINE]
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