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J Proteome Res. 2006 Feb;5(2):339-48.

Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase.

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  • 1Laboratory of Bioenergetics, Bât. B6c, Allée de la Chimie 3, 4000, Liège, Belgium.


The energy-dissipating alternative oxidase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.

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