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J Clin Microbiol. 2006 Feb;44(2):619-21.

High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

Author information

  • 1Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany.

Abstract

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

PMID:
16455927
[PubMed - indexed for MEDLINE]
PMCID:
PMC1392649
Free PMC Article
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