Vaccine. 2006 May 22;24(21):4671-6. Epub 2005 Aug 26.

pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine plasmids.

Williams JA, Luke J, Johnson L, Hodgson C.

Source

Nature Technology Corporation, 4701 Innovation Drive, Lincoln, NE 68521, USA. jim@natx.com

Abstract

DNA vaccines have the potential to provide a safe route for protective immunity to neoplasms and infectious agents. However, current DNA vaccine plasmids are not optimal with additional non-essential DNA, nor do they facilitate controlled or flexible targeting of antigens to various intracellular destinations. A family of DNA vaccine vectors, optimized and minimized to comply with FDA guidelines regarding content and elimination of extraneous materials, was constructed. The resulting vectors are much smaller than existing vectors, drive higher levels of target gene expression, facilitate high throughput cloning applications, and allow simultaneous cloning into multiple vectors that feature various intracellular targeting destinations for the protein product. The ability to control expression and trafficking is intended to provide a rapid, rational approach to cancer therapy and emerging infectious diseases.

PMID:: 16448726; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells. [Methods Mol Biol. 2007]
Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells.
Cheng TL, Chang WT. Methods Mol Biol. 2007; 408:223-41.
Review Technical issues in construction of nucleic acid vaccines. [Methods. 2003]
Review Technical issues in construction of nucleic acid vaccines.
Ertl PF, Thomsen LL. Methods. 2003 Nov; 31(3):199-206.
Review DNA vaccine design. [Methods Mol Med. 2006]
Review DNA vaccine design.
Brandsma JL. Methods Mol Med. 2006; 127:3-10.
Expanding the repertoire of Modified Vaccinia Ankara-based vaccine vectors via genetic complementation strategies. [PLoS One. 2009]
Expanding the repertoire of Modified Vaccinia Ankara-based vaccine vectors via genetic complementation strategies.
Garber DA, O'Mara LA, Zhao J, Gangadhara S, An I, Feinberg MB. PLoS One. 2009; 4(5):e5445. Epub 2009 May 6.
Engineering enhancement of the immune response to HBV DNA vaccine in mice by the use of LIGHT gene adjuvant. [J Virol Methods. 2008]
Engineering enhancement of the immune response to HBV DNA vaccine in mice by the use of LIGHT gene adjuvant.
Zhang Y, Jiang W, Fan Y, Wen J, Hao W, Qian M. J Virol Methods. 2008 Nov; 153(2):142-8. Epub 2008 Sep 3.

See reviews... See all...

Cited by 6 PubMed Central articles

Generation of multivirus-specific T cells to prevent/treat viral infections after allogeneic hematopoietic stem cell transplant. [J Vis Exp. 2011]
Generation of multivirus-specific T cells to prevent/treat viral infections after allogeneic hematopoietic stem cell transplant.
Gerdemann U, Vera JF, Rooney CM, Leen AM. J Vis Exp. 2011 May 27; (51). Epub 2011 May 27.
Coexpressed RIG-I agonist enhances humoral immune response to influenza virus DNA vaccine. [J Virol. 2011]
Coexpressed RIG-I agonist enhances humoral immune response to influenza virus DNA vaccine.
Luke JM, Simon GG, Söderholm J, Errett JS, August JT, Gale M Jr, Hodgson CP, Williams JA. J Virol. 2011 Feb; 85(3):1370-83. Epub 2010 Nov 24.
Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields. [J Gene Med. 2010]
Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields.
Carnes AE, Luke JM, Vincent JM, Anderson S, Schukar A, Hodgson CP, Williams JA. J Gene Med. 2010 Oct; 12(10):818-31.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine...
pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine plasmids.
Vaccine. 2006 May 22 ;24(21):4671-6. Epub 2005 Aug 26 .

PubMed
Psychopathology of panic attacks in panic disorder.
Psychopathology of panic attacks in panic disorder.
J Affect Disord. 2006 May ;92(1):55-62. Epub 2006 Jan 31 .

PubMed
Benefits of prolonged gradient separation for high-performance liquid chromatogr...
Benefits of prolonged gradient separation for high-performance liquid chromatography-tandem mass spectrometry quantitation of plasma total 15-series F-isoprostanes.
Anal Biochem. 2006 Mar 1 ;350(1):41-51. Epub 2006 Jan 13 .

PubMed
Comprehensive comparative analysis of kinesins in photosynthetic eukaryotes.
Comprehensive comparative analysis of kinesins in photosynthetic eukaryotes.
BMC Genomics. 2006 Jan 31 ;7:18.

PubMed
Role of vitamin D in bone resorption.
Role of vitamin D in bone resorption.
J Cell Biochem. 1992 May ;49(1):53-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: