Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Arch Phys Med Rehabil. 2006 Feb;87(2):241-6.

The effect of 30 minutes of passive stretch of the rat soleus muscle on the myogenic differentiation, myostatin, and atrogin-1 gene expressions.

Author information

  • 1Unit of Skeletal Muscle Plasticity, Department of Physical Therapy, Federal University of São Carlos, Brazil.

Abstract

The effect of 30 minutes of passive stretch of the rat soleus muscle on the myogenic differentiation, myostatin, and atrogin-1 gene expressions.

OBJECTIVE:

To evaluate the effect of passive stretch, applied for 30 minutes to the rat soleus muscle, on the myogenic differentiation (myoD), myostatin, and atrogin-1 gene expressions.

DESIGN:

Case-controlled study.

SETTING:

University laboratory.

ANIMALS:

Fifty 12-week-old male Wistar rats.

INTERVENTIONS:

Six groups of animals were given a single stretch bout and were evaluated immediately and 8, 24, 48, 72, and 168 hours later. Another 3 groups were evaluated immediately after 2, 3, and 7 stretches. An intact control group was also analyzed.

MAIN OUTCOME MEASURES:

The messenger ribonucleic acid (mRNA) levels of myoD, myostatin, and atrogin-1 were assessed by real-time polymerase chain reaction.

RESULTS:

Twenty-four hours after a single session of stretch only, the myoD mRNA levels had increased compared with the control group, whereas an increase in the atrogin-1 expression was observed after 2, 3, and 7 stretches.

CONCLUSIONS:

A single session of passive stretch increased the myoD gene expression, a factor related to muscle growth. Interestingly, daily stretches increased the atrogin-1 gene expression, a gene primarily associated with muscle atrophy. The results indicated that gene expression was responsive to the number of stretch sessions.

PMID:
16442979
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk