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Genes Dev. 2006 Jan 15;20(2):153-8.

Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression.

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  • 1Banting and Best Department of Medical Research, University of Toronto, Ontario, M5G 1L6, Canada.

Abstract

Sequence-based analyses have predicted that approximately 35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.

PMID:
16418482
[PubMed - indexed for MEDLINE]
PMCID:
PMC1356107
Free PMC Article
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