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Methods Enzymol. 2005;404:1-8.

Arrays of transfected mammalian cells for high content screening microscopy.

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  • 1Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

In this chapter we describe protocols for reverse transfection to generate mammalian cell arrays for systematic gene knock-downs by RNAi or knock-ins by ectopic cDNA expression. The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel.

PMID:
16413252
[PubMed - indexed for MEDLINE]
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