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Methods Enzymol. 2005;404:1-8.

Arrays of transfected mammalian cells for high content screening microscopy.

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  • 1Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany.


In this chapter we describe protocols for reverse transfection to generate mammalian cell arrays for systematic gene knock-downs by RNAi or knock-ins by ectopic cDNA expression. The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel.

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