Send to:

Choose Destination
See comment in PubMed Commons below
J Am Chem Soc. 2006 Jan 11;128(1):24-5.

A dual parameter FRET probe for measuring PKC and PKA activity in living cells.

Author information

  • 1European Molecular Biology Institute (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.


Cell function is regulated by complex and often interdependent networks of signaling molecules. To accurately describe these networks, it is important to monitor multiple signals in parallel. To this end, we have developed a genetically encoded, FRET-based probe that independently monitors both protein kinase A (PKA) and protein kinase C (PKC) activity in vivo. Artificial as well as physiological stimulants produced a negative or positive change in FRET efficiency following PKA or PKC activation, respectively. Mutations of the phosphate-accepting amino acids of the PKC substrate yielded a probe that was sensitive to PKA activation alone.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk