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1: J Chromatogr A. 2006 Feb 3;1104(1-2):263-71. Epub 2005 Dec 27.Click here to read Links

Separation of cationic aracyl derivatives of betaines and related compounds.

Storer MK, McEntyre CJ, Lever M.

Biochemistry Unit, Canterbury Health Laboratories, P.O. Box 151, Christchurch, New Zealand.

Cationic aracyl esters of betaines can be formed by alkylation with aracyl halides or trifluoromethanesulfonates. HPLC on a non-endcapped strong cation exchange (SCX) column gave high retention of these derivatives. Cation exchange HPLC may be carried out on a normal-phase (silica or alumina) column using a polar organic solvent (acetonitrile, propan-2-ol) containing an aqueous buffer with an organic cation and a hydrophilic anion. Selectivity is affected by the choice of organic solvent and buffer, e.g. alcohols decrease the retention times of hydroxybetaines such as carnitine. Retention is reduced by increasing the water content and the buffer concentration. Capillary electrophoresis migration times are affected by the choice of buffer anion, with low pH citrate buffers favoured.

PMID: 16376895 [PubMed - indexed for MEDLINE]

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