FEBS Lett. 2006 Jan 23;580(2):450-4. Epub 2005 Dec 20.

Elasticity and adhesion of resting and lipopolysaccharide-stimulated macrophages.

Leporatti S, Gerth A, Köhler G, Kohlstrunk B, Hauschildt S, Donath E.

Source

Institute of Medical Physics and Biophysics, University of Leipzig, D-04107 Leipzig, Saxony, Germany. leps@medizin.uni-leipzig.de

Abstract

Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS-stimulated cells (separation force=37+/-3 nN) was almost twice as high as that of resting macrophages (16+/-1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young's modulus K, a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.

PMID:: 16376879; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Lipopolysaccharides

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