Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2006 Jan;3(1):23-5.

Fluorescent sensors for rapid monitoring of intracellular cGMP.

Author information

  • 1Institute of Pharmacology and Toxicology, Versbacher Str. 9, D-97078 W├╝rzburg, Germany.


Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.

Comment in

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk