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J Zhejiang Univ Sci B. 2006 Jan;7(1):28-33.

Promoter trapping in Magnaporthe grisea.

Liu XH, Lu JP, Wang JY, Min H, Lin FC.

Source

Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. minhang@zju.edu.cn

Abstract

Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

PMID:: 16365922; [PubMed - indexed for MEDLINE]
PMCID:: PMC1361756

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