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Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1228-42. Epub 2005 Dec 15.

Dystroglycan receptor is involved in integrin activation in intestinal epithelia.

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  • 1Department of Medicine, Division of Digestive Diseases, Emory University School of Medicine, Atlanta, Georgia, Atlanta, 30322, USA. dmerlin@emory.edu

Abstract

The dystroglycans (alpha-DG and beta-DG), which play important roles in the formation of basement membranes, have been well studied in skeletal muscle and nerve, but their expression and localization in intestinal epithelial cells has not been previously investigated. Here, we demonstrated that the DG complex, composed of alpha-DG, beta-DG, and utrophin, is specifically expressed in the basolateral membrane of the Caco-2-BBE monolayer. The DG complex coprecipitated with beta(1)-integrin, suggesting a possible interaction among these proteins. In addition, we observed that activation of DG receptors by laminin-1 enhanced the interaction between beta(1)-integrin and laminin-1, whereas activation of DG receptors by laminin-2 reduced the interaction between beta(1)-integrin and laminin-2. Finally, we demonstrated that the intracellular COOH-terminal tail of beta-DG and its binding to the DG binding domain of utrophin are crucial for the interactions between laminin-1/-2 and beta(1)-integrin. Collectively, these novel results indicate that dystroglycans play important roles in the regulation of interactions between intestinal epithelial cells and the extracellular matrix.

PMID:
16357060
[PubMed - indexed for MEDLINE]
PMCID:
PMC2738938
Free PMC Article
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