The candidate tumor suppressor CST6 alters the gene expression profile of human breast carcinoma cells: down-regulation of the potent mitogenic, motogenic, and angiogenic factor autotaxin

Biochem Biophys Res Commun. 2006 Feb 3;340(1):175-82. doi: 10.1016/j.bbrc.2005.11.171. Epub 2005 Dec 9.

Abstract

We recently coined CST6 as a novel candidate tumor suppressor gene for breast cancer. CST6 indeed is expressed in the normal human breast epithelium, but little or not at all in breast carcinomas and breast cancer cell lines. Moreover, ectopic expression of CST6 in human breast cancer cells suppressed cell proliferation, migration, invasion, and orthotopic tumor growth. To obtain insights into the molecular mechanism by which CST6 exhibits its pleiotropic effects on tumor cells, we compared global gene expression profiles in mock- and CST6-transfected human MDA-MB-435S cells. Out of 12,625 transcript species, 61 showed altered expression. These included genes for extracellular matrix components, cytokines, kinases, and phosphatases, as well as several key transcription factors. TaqMan PCR assays were used to confirm the microarray data for 7 out of 11 genes. One down-regulated gene product, secreted autotaxin/lyso-phospholipase D, was of particular interest because its down-regulation by CST6 could explain most of CST6's effect on the breast cancer cells. This study thus provides the first evidence that CST6 plays a role in the modulation of genes, particularly, genes that are highly relevant to breast cancer progression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / blood supply
  • Breast Neoplasms / metabolism*
  • Cell Line, Tumor
  • Cystatin M
  • Cystatins / metabolism*
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic*
  • Glucose-6-Phosphate Isomerase / metabolism*
  • Glycoproteins / metabolism*
  • Humans
  • Mitosis
  • Multienzyme Complexes / metabolism*
  • Neoplasm Proteins / metabolism*
  • Neovascularization, Pathologic / metabolism*
  • Phosphodiesterase I
  • Phosphoric Diester Hydrolases
  • Pyrophosphatases
  • Tumor Suppressor Proteins / metabolism*

Substances

  • CST6 protein, human
  • Cystatin M
  • Cystatins
  • Glycoproteins
  • Multienzyme Complexes
  • Neoplasm Proteins
  • Tumor Suppressor Proteins
  • Phosphoric Diester Hydrolases
  • Phosphodiesterase I
  • alkylglycerophosphoethanolamine phosphodiesterase
  • Pyrophosphatases
  • Glucose-6-Phosphate Isomerase