Send to:

Choose Destination
See comment in PubMed Commons below
Appl Environ Microbiol. 1988 Jun;54(6):1570-4.

Phospholipid Fatty Acid Composition of the Syntrophic Anaerobic Bacterium Syntrophomonas wolfei.

Author information

  • 1Agricultural Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Monticello, Florida 32344; Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 ; and Department of Biological Sciences, Florida State University, Tallahassee, Florida 32306.


The membrane phospholipid fatty acids (PLFAs) from several cocultures and a pure culture of Syntrophomonas wolfei were determined by capillary column gas chromatography. Cocultures of S. wolfei with a Desulfovibrio sp. contained PLFAs from both organisms, whereas PLFAs from a coculture with Methanospirillum hungatei contained very little biomass to analyze. The pure culture of S. wolfei grown on crotonate provided the best material for analysis of the PLFAs. The predominant PLFAs of S. wolfei were the monounsaturated 16:1omega7c and 16:1omega9c and the saturated 16:0 and 14:0. A low concentration of the diunsaturated 18:2omega6 was detected. The PLFA analysis provides additional information for consideration in the determination of the profile of PLFAs obtained from anaerobic environments. In addition, this information may aid in the understanding of the physiology and phylogeny of S. wolfei and other syntrophic bacteria.

Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk