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Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18252-7. Epub 2005 Dec 12.

Volumetric tomography of fluorescent proteins through small animals in vivo.

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  • 1Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Harvard Medical School, Charlestown, MA 02129, USA.


Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.

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