Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda)

Mar Pollut Bull. 2005;51(8-12):757-68. doi: 10.1016/j.marpolbul.2005.02.014. Epub 2005 Apr 18.

Abstract

The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using lambdaZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Copepoda / genetics*
  • DNA Primers
  • Environmental Monitoring / methods*
  • Expressed Sequence Tags*
  • Gene Expression Profiling
  • Gene Expression*
  • Gene Library

Substances

  • DNA Primers