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Wei Sheng Wu Xue Bao. 2003 Feb;43(1):79-86.

[Selection of A strain producing beta-lactam antibiotics acylases].

[Article in Chinese]

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  • 1Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for, Biological Sciences, Chinese Academy of Sciences, China.


A series of substrates analogues containing the same or similar side chain of its substrate have been synthesized and applied to screen cephalosporin acylase producers from a mass of microorganisms. The deacylation products of these analogues can be detected conveniently and used as screening indicators for the cephalosporin-acylase-producing-microorganism. Six strains possessing deacylation activity have been screened out with these substrate analogs. Among them, strain ZH0650 can simultaneously hydrolyze GL-7ACA, NIPAB and other analogues including AD-NABA. Further investigation on this strain confirmed that it could produce at least three acylases, ADNABA acylase, penicillin G acylase and cephalosporin acylase, which were characterized by bioassay with multiple substrate analogues. This is the first report that three different acylases were produced by one strain.

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