WNK3 kinase regulates the renal NCC and NKCC2 cotransporters. (A) WNK3 is an active kinase. Wild-type WNK3 (WT), kinase-dead WNK3 (kin-), and PHAII-like mutant WNK3 constructs were tagged with HA and expressed in COS-7 cells, purified by immunoprecipitation, and incubated with γ-³²P ATP. The products were separated by SDS/PAGE, exposed to film, and also separately stained with anti-HA antibodies. Phosphorylated WNK3 (³²P-WNK3) species were seen at 200 kDa (the size of WNK3-HA) in assays with WT and PHAII-like mutant WNK3 but were markedly reduced in assays with kinase-dead WNK3. Western blots with anti-HA and IgG demonstrate equivalent protein loading. (B) WNK3 regulates NCC. Xenopus oocytes were injected with cRNAs encoding NCC alone or in combination with wild-type WNK3, kinase-dead WNK3 (kin-), or PHAII-like WNK3. Metolazone-sensitive ²²Na⁺ influx was measured as described in Methods. Results are expressed as mean ± SE of metolazone-sensitive ²²Na⁺ influx. ^*, P < 0.0001 vs. NCC alone. WNK3 markedly increases metolazone-sensitive ²²Na⁺ influx, kinase-dead WNK3 marked inhibits this activity, whereas PHAII-WNK3 behaves like wild-type WNK3. (C and D) WNK3 regulates NCC surface expression. (C) Oocytes were injected with cRNAs encoding EGFP-tagged NCC alone or in combination with wild-type or mutant WNK3. Surface expression of NCC was quantitated by confocal microscopy as described in Methods. Mean fluorescence seen in oocytes expressing GFP-NCC alone is expressed as 100%; other groups are expressed as a percentage of this value. Effects of WNK3 constructs on NCC surface expression closely parallel its effects on ²²Na⁺ flux. ^*, P < 10^-6 vs. NCC alone. (D) Examples of confocal microscopy of oocytes expressing GFP-tagged NCC alone or with wild-type or kinase-dead WNK3. (E) WNK3 regulates NKCC2. Oocytes were injected with cRNAs encoding NKCC2 alone or in combination with wild-type or mutant WNK3; bumetanide-sensitive ⁸⁶Rb⁺ influx was measured as described in Methods. Mean ± SE of bumetanide-sensitive ⁸⁶Rb⁺ influx is shown for each group in a representative experiment. As for NCC, WNK3 increases NKCC2 activity, kinase-dead WNK3 inhibits NKCC2, activity and PHAII-like WNK3 behaves like wild-type kinase.

Proposed role of WNK3 in the kidney. By regulating apical NaCl entry in the TAL and DCT of the nephron, WNK3 could modulate NaCl and water reabsorption and therefore blood pressure. Kinase-active WNK3 might increase NaCl reabsorption, whereas kinase-inactive WNK3 (WNK3 kin-) might inhibit NaCl reabsorption. These active/inactive states of WNK3 may be achieved dynamically by ligands (e.g., downstream of vasopressin) binding to or dissociating from the kinase.

WNK3 is expressed at intercellular junctions along the nephron. Frozen mouse kidney sections were stained with anti-WNK3 antibody as described in Methods and visualized with immunofluorescence light microscopy. (A) WNK3 is present in all nephron segments. Low-power view of renal tubules in cross section stained with anti-WNK3 antibody reveals expression of WNK3 in all nephron segments. It is apparent that WNK3 predominantly localizes to intercellular junctions. (Original magnification, ×200.) (B) WNK3 expression in PCT. Tubule segments were stained with anti-WNK3 antibody and identified by costaining adjacent sections with anti-Megalin antibody, a PCT marker (not shown). (Original magnification, ×630.) (C-E) WNK3 (red) and ZO-1 (green) immunostaining in the TAL. Tubule segments were determined by costaining experiments with anti-WNK3 and anti-Tamm-Horsfall protein, a TAL marker (data not shown). WNK3 staining overlaps with ZO-1, a marker of tight junctions. (Original magnification, ×630.) (F) WNK3 expression in the DCT and connecting tubule (CNT), as determined by costaining experiments with anti-WNK3 and anti-Calbindin D-28K, a DCT/CNT marker (data not shown). (Original magnification, ×400.) (G) WNK3 expression in the cortical CD (CCD), determined by costaining with anti-WNK3 (red) and anti-Aquaporin-2 (AQP2), a CCD marker (blue). (Original magnification, ×630.) (H) WNK3 (red) and ZO-1 (green) immunostaining in the CCD. Tubule segments were determined by costaining experiments with anti-WNK3 and anti-AQP2. WNK3 staining overlaps with ZO-1, a marker of tight junctions. (Original magnification, ×700.)

WNK3 modulates the phosphorylation of NKCC2. Xenopus oocytes were injected with the indicated constructs and incubated at varying extracellular osmolarities as indicated. After incubation oocytes were lysed and Western blotting was performed by using the R5 (anti-phospho-NKCC2) or T9 (anti-NKCC2) antibodies as described in Methods. Phosphorylation of NKCC2 normally increases from negligible levels under hypotonic conditions (200 mM) to complete phosphorylation under hypertonic conditions (380 mM). In contrast, coexpression of NKCC2 with kinase-active WNK3 results in robust phosphorylation of NKCC2 at all osmolarities. Expression of kinase-dead WNK3 results in marked reduction of NKCC2 phosphorylation under hyperosmolar conditions.