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An analysis of the feasibility of short read sequencing.
School of Chemistry, University of Southampton, Southampton SO17 1BJ, UK.
Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.
PMID: 16275781 [PubMed - indexed for MEDLINE]
PMCID: PMC1278949
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Cited by 11 PubMed Central articles
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Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template.
Mir KU, Qi H, Salata O, Scozzafava G.
Nucleic Acids Res. 2009 Jan; 37(1):e5. Epub 2008 Nov 16.
[Nucleic Acids Res. 2009]
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Sequence space coverage, entropy of genomes and the potential to detect non-human DNA in human samples.
Liu Z, Venkatesh SS, Maley CC.
BMC Genomics. 2008 Oct 30; 9:509. Epub 2008 Oct 30.
[BMC Genomics. 2008]
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The long march: a sample preparation technique that enhances contig length and coverage by high-throughput short-read sequencing.
Sorber K, Chiu C, Webster D, Dimon M, Ruby JG, Hekele A, DeRisi JL.
PLoS One. 2008; 3(10):e3495. Epub 2008 Oct 22.
[PLoS One. 2008]
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