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1: Plant Methods. 2005 Sep 5;1(1):5.Click here to read Click here to read Links

Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat.

Jones HD, Doherty A, Wu H.

CPI Division, Rothamsted Research, Harpenden, AL5 2JQ, UK. huw.jones@bbsrc.ac.uk

Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

PMID: 16270934 [PubMed]

PMCID: PMC1277018