Abstract
A variety of furan-modified nucleoside phosphoramidite monomers has been prepared and efficiently incorporated into oligonucleotides. These take part in Diels-Alder reactions with fluorescent maleimides to give fluorescent-labeled oligonucleotides. This represents a strategy for oligonucleotide labeling that is orthogonal to amine-based methods.
MeSH terms
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Amines / chemistry
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Deoxycytidine / chemistry
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Fluorescent Dyes / pharmacology
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Furans / chemistry
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Hydrogen-Ion Concentration
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Macromolecular Substances
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Models, Chemical
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Molecular Biology / methods*
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Nucleosides / chemistry*
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Nucleotides / chemistry
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Oligodeoxyribonucleotides / chemistry
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Oligonucleotides / chemistry*
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Organophosphorus Compounds / chemistry*
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Temperature
Substances
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Amines
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Fluorescent Dyes
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Furans
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Macromolecular Substances
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Nucleosides
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Nucleotides
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Oligodeoxyribonucleotides
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Oligonucleotides
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Organophosphorus Compounds
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phosphoramidite
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Deoxycytidine
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furan