Send to:

Choose Destination
See comment in PubMed Commons below
FEMS Microbiol Lett. 2005 Dec 15;253(2):201-5. Epub 2005 Oct 7.

Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene.

Author information

  • 1Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark.


An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ngml(-1) to 16 microgml(-1), which represents a significant improvement of the original version.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk