J Biosci Bioeng. 2004;98(2):140-3.

Systematic transient assays of promoter activities for leaf-specific genes identified by gene-expression profiling with cDNA microarrays in Arabidopsis thaliana.

Yamakawa S, Ando K, Chisaka A, Yoshida K, Shinmyo A, Kohchi T.

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Technology Department Research Association for Biotechnology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan. s-yamaka@bs.naist.jp

Abstract

We identified 85 genes highly expressed in leaves using an Arabidopsis cDNA microarray. A vector, pRAB5, was designed to allow cloning and assaying of the promoters. Fifty-one promoters from the selected genes were cloned and then assayed using a microprojectile bombardment and dual luciferase reporter assay system. This system allowed efficient systematic assays of promoter activity.

PMID:: 16233681; [PubMed]

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Systematic transient assays of promoter activities for leaf-specific genes identified by gene-expression profiling with cDNA microarrays in Arabidopsis thaliana.

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