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Nat Rev Mol Cell Biol. 2005 Sep;6(9):702-14.

Proteomics of organelles and large cellular structures.

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  • 1Department of Cell Biology, 10550 North Torrey Pines Road, The Scripps Research Institute, La Jolla, California 92037, USA. jyates@scripps.edu


The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights into the biology and functions of these structures. Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies.

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