Inhibitory effects of coumarin and acetylene constituents from the roots of Angelica furcijuga on D-galactosamine/lipopolysaccharide-induced liver injury in mice and on nitric oxide production in lipopolysaccharide-activated mouse peritoneal macrophages

Bioorg Med Chem. 2006 Jan 15;14(2):456-63. doi: 10.1016/j.bmc.2005.08.038. Epub 2005 Oct 12.

Abstract

The methanolic extract (200 mg/kg, p.o. and i.p.), principal coumarin constituents (isoepoxypteryxin, anomalin, and praeroside IV), and a polyacetylene constituent (falcarindiol) (25 mg/kg, i.p.) from the roots of Angelica furcijuga protected the liver injury induced by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) in mice. In in vitro experiments, coumarin constituents (hyuganins A-D, anomalin, pteryxin, isopteryxin, and suksdorfin) and polyacetylene constituents [(-)-falcarinol and falcarindiol] substantially inhibited LPS-induced NO and/or TNF-alpha production in mouse peritoneal macrophages, and isoepoxypteryxin inhibited D-GalN-induced cytotoxicity in primary cultured rat hepatocytes. Furthermore, hyuganin A, anomalin, and isopteryxin inhibited the decrease in cell viability by TNF-alpha in L929 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylene / pharmacology*
  • Angelica / chemistry*
  • Animals
  • Cells, Cultured
  • Coumarins / pharmacology*
  • Galactosamine / toxicity*
  • Lipopolysaccharides / toxicity*
  • Liver / drug effects*
  • Macrophage Activation
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / metabolism
  • Male
  • Mice
  • Nitric Oxide / biosynthesis*
  • Plant Roots / chemistry*
  • Rats
  • Rats, Wistar
  • Tumor Necrosis Factors / biosynthesis

Substances

  • Coumarins
  • Lipopolysaccharides
  • Tumor Necrosis Factors
  • Nitric Oxide
  • Galactosamine
  • Acetylene