Format

Send to:

Choose Destination
See comment in PubMed Commons below
Clin Hemorheol Microcirc. 2005;33(3):253-61.

Role of Ca2+ in the effects of shear stress and TNF-alpha on caveolin-1 expression.

Author information

  • 1Cell and Tissue Engineering and Mechanics, LEMTA-UMR CNRS 7563, Faculté de Médecine Plateau de Brabois, 54500 Vandoeuvre lès Nancy, France.

Abstract

The aim of this work was to study the influences of Ca2+ (medium free calcium, with BAPTA, with 100 mM Ca2+, 100 mM Ca2++10 microM ionomycin) on the expression of caveolin-1 (structural protein of caveolae) of endothelial cells (ECs) submitted to mechanical forces (shear stress) or biochemical stimulation (TNF-alpha). We found that shear stress enhanced the caveolin-1 expression. Simultaneously, the caveolin-1 expression is dependant on [Ca2+]i: [Ca2+]free medium+100 microM BAPTA<[Ca2+]free medium<[Ca2+]100 mM<[Ca2+]100 mM+10 microM ionomycin. In contrast, TNF-alpha induced a decrease of caveolin-1 in the cells, whatever the [Ca2+]i. These results suggest that there could be a synergistic effect between shear stress and Ca2+ on caveolin-1 expression but an inflammatory stimulation (TNF-alpha) induces a down regulation of caveolin-1 expression.

PMID:
16215291
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Miscellaneous

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for IOS Press
    Loading ...
    Write to the Help Desk