J Immunol. 2005 Oct 15;175(8):5314-23.

Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.

Source

Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Abstract

As pattern recognition receptors, TLRs signal and induce expression of multiple host defense genes including proinflammatory cytokines and chemokines. To investigate the mechanisms of up-regulation of proinflammatory cytokines and chemokines during SIV infection in rhesus macaques, we measured the relative levels of expression of TLRs 1-10 in lymphoid tissues during different stages of SIV infection. By real-time RT-PCR, TLR3 was determined to be up-regulated in macaque lymph nodes (LN) throughout the course of infection, whereas TLR9 was down-regulated during early stages of infection. CXCL9/Mig, CXCL10/IP-10, IFN-gamma, and IFN-alpha mRNAs were also increased during acute SIV infection and AIDS. Treatment of macaque spleen and LN cells with TLR3 and TLR9 ligands led to the induction of these same genes. TLR3 stimulation had disparate effects on viral transcription and viral replication, because poly(I:C), a model TLR3 ligand, stimulated the viral promoter but potently inhibited SIV replication in primary cultures of macaque spleen and LN cells. These findings identify roles for TLR3 inflammation in lymphoid tissues and in the immunopathogenesis of HIV-1/SIV, and suggest that TLR3 ligands could potentially be used to flush out latently infected cells that persist during antiretroviral therapies.

PMID:: 16210637; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

HL62056/HL/NHLBI NIH HHS/United States

LinkOut - more resources

Full Text Sources

HighWire Press

Medical

Immunopathogenesis - HIV InSite

Supplemental Content

Related citations

Increased expression of the inflammatory chemokine CXC chemokine ligand 9/monokine induced by interferon-gamma in lymphoid tissues of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome. [Blood. 2002]
Increased expression of the inflammatory chemokine CXC chemokine ligand 9/monokine induced by interferon-gamma in lymphoid tissues of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome.
Reinhart TA, Fallert BA, Pfeifer ME, Sanghavi S, Capuano S 3rd, Rajakumar P, Murphey-Corb M, Day R, Fuller CL, Schaefer TM. Blood. 2002 May 1; 99(9):3119-28.
Expression of IFN-gamma induced CXCR3 agonist chemokines and compartmentalization of CXCR3+ cells in the periphery and lymph nodes of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome. [J Med Primatol. 2003]
Expression of IFN-gamma induced CXCR3 agonist chemokines and compartmentalization of CXCR3+ cells in the periphery and lymph nodes of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome.
Sarkar S, Kalia V, Murphey-Corb M, Montelaro RC, Reinhart TA. J Med Primatol. 2003 Aug; 32(4-5):247-64.
The relationship between simian immunodeficiency virus RNA levels and the mRNA levels of alpha/beta interferons (IFN-alpha/beta) and IFN-alpha/beta-inducible Mx in lymphoid tissues of rhesus macaques during acute and chronic infection. [J Virol. 2002]
The relationship between simian immunodeficiency virus RNA levels and the mRNA levels of alpha/beta interferons (IFN-alpha/beta) and IFN-alpha/beta-inducible Mx in lymphoid tissues of rhesus macaques during acute and chronic infection.
Abel K, Alegria-Hartman MJ, Rothaeusler K, Marthas M, Miller CJ. J Virol. 2002 Aug; 76(16):8433-45.
Review Activation of the blood-brain barrier by SIV (simian immunodeficiency virus) requires cell-associated virus and is not restricted to endothelial cell activation. [Biochem Soc Trans. 2004]
Review Activation of the blood-brain barrier by SIV (simian immunodeficiency virus) requires cell-associated virus and is not restricted to endothelial cell activation.
MacLean AG, Rasmussen TA, Bieniemy D, Lackner AA. Biochem Soc Trans. 2004 Nov; 32(Pt 5):750-2.
Review Toll-like receptors: expression and involvement in multiple myeloma. [Leuk Res. 2010]
Review Toll-like receptors: expression and involvement in multiple myeloma.
Chiron D, Jego G, Pellat-Deuceunynck C. Leuk Res. 2010 Dec; 34(12):1545-50. Epub 2010 Jul 1.

See reviews... See all...

Cited by 9 PubMed Central articles

Interferons induce CXCR3-cognate chemokine production by human metastatic melanoma. [J Immunother. 2010]
Interferons induce CXCR3-cognate chemokine production by human metastatic melanoma.
Dengel LT, Norrod AG, Gregory BL, Clancy-Thompson E, Burdick MD, Strieter RM, Slingluff CL Jr, Mullins DW. J Immunother. 2010 Nov-Dec; 33(9):965-74.
Simian immunodeficiency virus infection alters chemokine networks in lung tissues of cynomolgus macaques: association with Pneumocystis carinii infection. [Am J Pathol. 2010]
Simian immunodeficiency virus infection alters chemokine networks in lung tissues of cynomolgus macaques: association with Pneumocystis carinii infection.
Qin S, Fallert Junecko BA, Trichel AM, Tarwater PM, Murphey-Corb MA, Kirschner DE, Reinhart TA. Am J Pathol. 2010 Sep; 177(3):1274-85. Epub 2010 Jul 29.
Nonpathogenesis of simian immunodeficiency virus infection is associated with reduced inflammation and recruitment of plasmacytoid dendritic cells to lymph nodes, not to lack of an interferon type I response, during the acute phase. [J Virol. 2010]
Nonpathogenesis of simian immunodeficiency virus infection is associated with reduced inflammation and recruitment of plasmacytoid dendritic cells to lymph nodes, not to lack of an interferon type I response, during the acute phase.
Campillo-Gimenez L, Laforge M, Fay M, Brussel A, Cumont MC, Monceaux V, Diop O, Lévy Y, Hurtrel B, Zaunders J, et al. J Virol. 2010 Feb; 84(4):1838-46. Epub 2009 Nov 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus...
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
J Immunol. 2005 Oct 15 ;175(8):5314-23.

PubMed
Recombinant plasmid associated cell aggregation and high-frequency conjugation o...
Recombinant plasmid associated cell aggregation and high-frequency conjugation of Streptococcus lactis ML3.
J Bacteriol. 1981 Jun ;146(3):937-44.

PubMed
Sensitive, specific polymorphism discovery in bacteria using massively parallel ...
Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
Nat Methods. 2009 Jan ;6(1):67-9. Epub 2008 Dec 14 .

PubMed
Integration of biological networks and gene expression data using Cytoscape.
Integration of biological networks and gene expression data using Cytoscape.
Nat Protoc. 2007 ;2(10):2366-82.

PubMed
Clonal vaccinia virus grown in cell culture fully protects monkeys from lethal m...
Clonal vaccinia virus grown in cell culture fully protects monkeys from lethal monkeypox challenge.
Vaccine. 2008 Jan 24 ;26(4):581-8. Epub 2007 Nov 20 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: