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Zhong Yao Cai. 2005 Jun;28(6):482-5.

[The modulating of aloe polysaccharides on the cell cycle and cycle regulating protein expression in X-ray irradiated non-malignant cells].

[Article in Chinese]

Author information

  • 1Guangzhou University of TCM School of Chinese Materia Medica, Guangzhou.

Abstract

OBJECTIVE:

To study the modulating of Aloe Polysaccharides on the cell cycle and cycle regulating protein expression in X-ray irradiated non-malignant cells.

METHODS:

The cell cycle was analyzed by flow cytometric analyzed. The levels of cell cycle regulating protein expression were tested by Western blot.

RESULTS:

A distinct G2/M block happened in 293 and C. Liver cells after irradiation. The pre-treatment of AP in the concentration of 50 microg/ml caused an increasing G0/G1 phase population and decreasing G2/M phase population. Meanwhile, pre-treatment of AP could significantly decrease the high expression of p53 protein caused by irradiation and evidently enhance the expression of P21, Cyclin B1 and pRb protein. Pre-treatment of AP had no evident effect on p27,CDK4 and Cyclin D1 protein.

CONCLUSION:

There is a radioprotective effect of AP on non-malignant cells. This effect is related to alleviating the cell cycle turbulence. The modulating of Aloe Polysaccharides on the cell cycle regulating protein expression in X-ray irradiated non-malignant cells contributes to its alleviating effect on the cell cycle turbulence.

PMID:
16209264
[PubMed - indexed for MEDLINE]
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