Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing

Gannon C Mak; P L Ho; Cindy W S Tse; Susanna K P Lau; Samson S Y Wong

doi:10.1128/JCM.43.10.5391-5392.2005

Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing

J Clin Microbiol. 2005 Oct;43(10):5391-2. doi: 10.1128/JCM.43.10.5391-5392.2005.

Authors

Gannon C Mak¹, P L Ho, Cindy W S Tse, Susanna K P Lau, Samson S Y Wong

Affiliation

¹ Centre of Infection and Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China.

Abstract

This paper reports the first case of Haemophilus quentini bacteremia with reduced susceptibility to levofloxacin and resistance to nalidixic acid identified by 16S rRNA sequencing. There was an S 84 L substitution in gyrA and an S 84 I substitution in par C. The isolate had co-resistance to ampicillin (beta-lactamase positive) and tetracycline mediated by the tet(B) gene.

Publication types

Case Reports

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteremia / microbiology*
Bacterial Typing Techniques
DNA, Bacterial / analysis
Drug Resistance, Multiple, Bacterial*
Female
Haemophilus / classification*
Haemophilus / drug effects
Haemophilus / genetics
Haemophilus / isolation & purification
Haemophilus Infections / microbiology
Humans
Infant, Newborn
Levofloxacin
Nalidixic Acid / pharmacology
Ofloxacin / pharmacology
Phenotype
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA*
Tetracycline Resistance

Substances

Anti-Bacterial Agents
DNA, Bacterial
RNA, Ribosomal, 16S
Nalidixic Acid
Levofloxacin
Ofloxacin