Format

Send to:

Choose Destination
See comment in PubMed Commons below
Ann Rheum Dis. 2006 May;65(5):612-6. Epub 2005 Sep 28.

The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.

Author information

  • 1Molecular Immunology Research Group, Research Institute in Healthcare Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton WV1 1SB, UK. h.ejtehadi@wlv.ac.uk

Abstract

OBJECTIVE:

To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease.

DESIGN:

A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis.

METHODS:

40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products.

RESULTS:

Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02).

CONCLUSION:

There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.

PMID:
16192292
[PubMed - indexed for MEDLINE]
PMCID:
PMC1798125
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk