Detection of CD5 in B-cell chronic lymphoproliferative diseases by flow cytometry: a strong expression in B-cell chronic lymphocytic leukemia

Acta Cir Bras. 2005:20 Suppl 1:101-7. doi: 10.1590/s0102-86502005000700018.

Abstract

Purpose: CD5 is a T cell marker, aberrantly express in B cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL). Other chronic B cell malignancies including hairy cell leukemia (HCL) and B cell prolymphocytic leukemia (B-PLL) are CD5 negative or express this antigen in a weak way. In this study, CD5 expression was investigated in leukemic cells from 42 patients with chronic B cell lymphoproliferative disease.

Methods: We studied the CD5 expression in leukemic cells from 42 patients with chronic B-cell malignancies by flow cytometry. Demographic features such as age, sex and clinical date were also analyzed.

Results: There were 22 males and 20 females. The immunophenotyping showed that 35 cases were B-CLL, 3 B-PLL and HCL and one patient was MCL. CD5 expression was present in all B-CLL and MCL. Low expression of CD5 was observed in one patient with B-PLL and negative in all cases of HCL.

Conclusion: Our date demonstrated that CD5 expression can help distinguish among B-CLL from HCL and B-PLL, but is similar expressed in MCL.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood
  • CD5 Antigens / blood*
  • Diagnosis, Differential
  • Female
  • Flow Cytometry / methods*
  • Humans
  • Immunophenotyping*
  • Leukemia, Hairy Cell / blood
  • Leukemia, Hairy Cell / diagnosis
  • Leukemia, Lymphocytic, Chronic, B-Cell / blood
  • Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis*
  • Lymphocyte Count
  • Lymphoma, B-Cell / blood
  • Lymphoma, B-Cell / diagnosis*
  • Lymphoma, Mantle-Cell / blood
  • Lymphoma, Mantle-Cell / diagnosis
  • Lymphoproliferative Disorders / blood
  • Lymphoproliferative Disorders / diagnosis*
  • Male
  • Middle Aged

Substances

  • Biomarkers, Tumor
  • CD5 Antigens