Cell Signal. 1992 Mar;4(2):145-51.

Importance of substrate conformation in the phosphorylation of chromatin-associated proteins by exogenous protein kinase C.

Source

School of Biological Sciences, Flinders University of South Australia, Adelaide.

Abstract

Protein kinase C (PKC)-mediated phosphorylation of chromatin-associated proteins was studied in vitro. HL-60 and HeLa nuclear proteins were notably unresponsive to exogenously added brain PKC. In contrast, 3T3 fibroblasts and lymphocytes from primary cultures exhibited PKC-dependent phosphorylation of chromatin-associated proteins when chromatin was induced to expand. Unexpanded nuclei in all cell lines were unresponsive. Responsiveness was particularly obvious in the decondensed chromatin of primary lymphocytes, where a large number of proteins were phosphorylated in response to exogenous PKC. DNAase-I and micrococcal nuclease strongly modulated these phosphorylation patterns indicating that the substrates were DNA-associated. It was concluded that although substrate conformation, i.e. condensation state, was the primary determining factor in control of PKC-dependent nuclear protein phosphorylation, different cell lines greatly differ in their overall responsiveness to exogenous PKC.

PMID:: 1616821; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

EBSCO

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Phosphorylation and chromatin mechanics: the central importance of substrate conformation in determining the patterns of HL-60 nuclear phosphorylation. [Biochem Biophys Res Commun. 1991]
Phosphorylation and chromatin mechanics: the central importance of substrate conformation in determining the patterns of HL-60 nuclear phosphorylation.
Testori A, Skinner JD, Murray AW, Burgoyne LA. Biochem Biophys Res Commun. 1991 Oct 15; 180(1):329-33.
Human OGG1 undergoes serine phosphorylation and associates with the nuclear matrix and mitotic chromatin in vivo. [Nucleic Acids Res. 2002]
Human OGG1 undergoes serine phosphorylation and associates with the nuclear matrix and mitotic chromatin in vivo.
Dantzer F, Luna L, Bjørås M, Seeberg E. Nucleic Acids Res. 2002 Jun 1; 30(11):2349-57.
Selective activation of phospholipase C gamma1 and distinct protein kinase C subspecies in intracellular signaling by hepatocyte growth factor/scatter factor in primary cultured rat neocortical cells. [J Neurochem. 1998]
Selective activation of phospholipase C gamma1 and distinct protein kinase C subspecies in intracellular signaling by hepatocyte growth factor/scatter factor in primary cultured rat neocortical cells.
Machide M, Kamitori K, Nakamura Y, Kohsaka S. J Neurochem. 1998 Aug; 71(2):592-602.
Activation of protein kinase C delta by all-trans-retinoic acid. [J Biol Chem. 2003]
Activation of protein kinase C delta by all-trans-retinoic acid.
Kambhampati S, Li Y, Verma A, Sassano A, Majchrzak B, Deb DK, Parmar S, Giafis N, Kalvakolanu DV, Rahman A, et al. J Biol Chem. 2003 Aug 29; 278(35):32544-51. Epub 2003 Jun 12.
Review Calcium-dependent cell death. Role of the endonuclease, protein kinase C, and chromatin conformation. [Ann N Y Acad Sci. 1992]
Review Calcium-dependent cell death. Role of the endonuclease, protein kinase C, and chromatin conformation.
McCabe MJ Jr, Nicotera P, Orrenius S. Ann N Y Acad Sci. 1992 Nov 21; 663:269-78.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Importance of substrate conformation in the phosphorylation of chromatin-associa...
Importance of substrate conformation in the phosphorylation of chromatin-associated proteins by exogenous protein kinase C.
Cell Signal. 1992 Mar ;4(2):145-51.

PubMed
Guided self-help for bulimia nervosa in primary care: a randomized controlled tr...
Guided self-help for bulimia nervosa in primary care: a randomized controlled trial.
Psychol Med. 2005 Sep ;35(9):1283-94.

PubMed
Overexpression of platelet-derived growth factor receptor alpha in breast cancer...
Overexpression of platelet-derived growth factor receptor alpha in breast cancer is associated with tumour progression.
Breast Cancer Res. 2005 ;7(5):R788-95. Epub 2005 Aug 1 .

PubMed
Somatic mutation and gain of copy number of PIK3CA in human breast cancer.
Somatic mutation and gain of copy number of PIK3CA in human breast cancer.
Breast Cancer Res. 2005 ;7(5):R609-16. Epub 2005 May 31 .

PubMed
Population-based laboratory surveillance for Giardia sp. and Cryptosporidium sp....
Population-based laboratory surveillance for Giardia sp. and Cryptosporidium sp. infections in a large Canadian health region.
BMC Infect Dis. 2005 Sep 16 ;5:72.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: