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J Biol Chem. 2005 Nov 25;280(47):39238-45. Epub 2005 Sep 13.

Glycine metabolism in intact leaves by in vivo 13C and 15N labeling.

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  • 1Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.

Abstract

Solid-state (13)C NMR measurements of intact soybean leaves labeled by (13)CO(2) (at subambient concentrations) show that excess glycine from the photorespiratory C(2) cycle (i.e. glycine not part of the production of glycerate in support of photosynthesis) is either fully decarboxylated or inserted as (13)C-labeled glycyl residues in proteins. This (13)C incorporation in leaf protein, which is uniformly (15)N labeled by (15)NH(4)(15)NO(3), occurs as soon as 2 min after the start of (13)CO(2) labeling. In those leaves with lower levels of available nitrogen (as measured by leaf nitrate and glutamine-glutamate concentrations), the excess glycine is used primarily as glycyl residues in protein.

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