Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Am Chem Soc. 2005 Sep 21;127(37):12772-3.

Molecular assembly of superquenchers in signaling molecular interactions.

Author information

  • 1Center for Research at Bio/nano Interface, Department of Chemistry, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, Florida 32611, USA.

Abstract

We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. Our molecular assembly approach can effectively improve the sensitivity of a variety of fluorescent assays and can be widely useful for molecular interaction studies.

PMID:
16159250
[PubMed - indexed for MEDLINE]
PMCID:
PMC2536687
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk