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Microb Cell Fact. 2005 Sep 12;4:27.

Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins.

Author information

  • 1Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. Elena.Garcia.Fruitos@uab.es

Abstract

BACKGROUND:

Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein.

RESULTS:

By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis.

CONCLUSION:

This observation offers promising possibilities for the exploration of inclusion bodies as catalysts for industrial purposes, without any previous protein-refolding step.

PMID:
16156893
[PubMed]
PMCID:
PMC1224866
Free PMC Article

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