Direct isolation of satellite cells for skeletal muscle regeneration

Science. 2005 Sep 23;309(5743):2064-7. doi: 10.1126/science.1114758. Epub 2005 Sep 1.

Abstract

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / analysis
  • Cell Separation*
  • Cells, Cultured
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / biosynthesis
  • Diaphragm
  • Dystrophin / biosynthesis
  • Flow Cytometry
  • Green Fluorescent Proteins / analysis
  • Homeodomain Proteins / analysis
  • Homeodomain Proteins / biosynthesis
  • Mice
  • Mice, Inbred mdx
  • Mice, Nude
  • Muscle Development
  • Muscle Fibers, Skeletal / metabolism
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / physiology*
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Paired Box Transcription Factors
  • Regeneration*
  • Satellite Cells, Skeletal Muscle / cytology*
  • Satellite Cells, Skeletal Muscle / physiology
  • Satellite Cells, Skeletal Muscle / transplantation
  • Stem Cell Transplantation
  • Transcription Factors / analysis
  • Transcription Factors / biosynthesis

Substances

  • Antigens, CD34
  • DNA-Binding Proteins
  • Dystrophin
  • Homeodomain Proteins
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Paired Box Transcription Factors
  • Pax7 protein, mouse
  • Transcription Factors
  • Pax3 protein, mouse
  • Green Fluorescent Proteins