Abstract
Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigens, CD34 / analysis
-
Cell Separation*
-
Cells, Cultured
-
DNA-Binding Proteins / analysis
-
DNA-Binding Proteins / biosynthesis
-
Diaphragm
-
Dystrophin / biosynthesis
-
Flow Cytometry
-
Green Fluorescent Proteins / analysis
-
Homeodomain Proteins / analysis
-
Homeodomain Proteins / biosynthesis
-
Mice
-
Mice, Inbred mdx
-
Mice, Nude
-
Muscle Development
-
Muscle Fibers, Skeletal / metabolism
-
Muscle, Skeletal / cytology
-
Muscle, Skeletal / physiology*
-
PAX3 Transcription Factor
-
PAX7 Transcription Factor
-
Paired Box Transcription Factors
-
Regeneration*
-
Satellite Cells, Skeletal Muscle / cytology*
-
Satellite Cells, Skeletal Muscle / physiology
-
Satellite Cells, Skeletal Muscle / transplantation
-
Stem Cell Transplantation
-
Transcription Factors / analysis
-
Transcription Factors / biosynthesis
Substances
-
Antigens, CD34
-
DNA-Binding Proteins
-
Dystrophin
-
Homeodomain Proteins
-
PAX3 Transcription Factor
-
PAX7 Transcription Factor
-
Paired Box Transcription Factors
-
Pax7 protein, mouse
-
Transcription Factors
-
Pax3 protein, mouse
-
Green Fluorescent Proteins