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Science. 2005 Sep 23;309(5743):2064-7. Epub 2005 Sep 1.

Direct isolation of satellite cells for skeletal muscle regeneration.

Author information

  • 1CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France. dmontarr@pasteur.fr

Abstract

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

PMID:
16141372
[PubMed - indexed for MEDLINE]
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