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Science. 2005 Sep 2;309(5740):1577-81.

Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA.

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  • 1Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.

Abstract

MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5' noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.

PMID:
16141076
[PubMed - indexed for MEDLINE]
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