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RNA. 2005 Oct;11(10):1555-62. Epub 2005 Aug 30.

Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.

Author information

  • 1Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78751, USA.

Abstract

We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis.

PMID:
16131588
[PubMed - indexed for MEDLINE]
PMCID:
PMC1370839
Free PMC Article

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