Molecular recognition of nucleic acids: coralyne binds strongly to poly(A)

Feifei Xing; Guangtao Song; Jinsong Ren; Jonathan B Chaires; Xiaogang Qu

doi:10.1016/j.febslet.2005.07.091

Molecular recognition of nucleic acids: coralyne binds strongly to poly(A)

FEBS Lett. 2005 Sep 12;579(22):5035-9. doi: 10.1016/j.febslet.2005.07.091.

Authors

Feifei Xing¹, Guangtao Song, Jinsong Ren, Jonathan B Chaires, Xiaogang Qu

Affiliation

¹ Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

PMID: 16125177
DOI: 10.1016/j.febslet.2005.07.091

Abstract

The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant (Ka) of (1.8+/-0.3) x 10(6)M(-1). Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 degrees C, for the conditions of our study.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adenosine / chemistry
Adenosine / metabolism*
Animals
Berberine Alkaloids / metabolism*
Circular Dichroism
Macromolecular Substances
Molecular Structure
Nucleic Acid Conformation
Polymers / chemistry
Polymers / metabolism*
RNA, Messenger / metabolism

Substances

Berberine Alkaloids
Macromolecular Substances
Polymers
RNA, Messenger
polyadenosine
coralyne
Adenosine

Grants and funding

CA35635/CA/NCI NIH HHS/United States