Abstract
Phosphatidylinositol 3,4,5-trisphosphate is a major intracellular messenger molecule thought to be formed almost exclusively by cytosolic, wortmannin-inhibited phosphoinositide 3-kinase family members. Inositol polyphosphate multikinase was identified as an enzyme that generates a series of water-soluble inositol phosphates. We now report the robust, physiologic, and evolutionarily conserved phosphoinositide 3-kinase activity of inositol polyphosphate multikinase, which is localized to nuclei and unaffected by wortmannin. In yeast, this inositol lipid kinase activity physiologically regulates transcription.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Androstadienes / pharmacology
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Animals
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Cell Line
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Cell Nucleus / enzymology*
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Cell Nucleus / genetics
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Chlorocebus aethiops
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Gene Expression Regulation*
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Humans
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Phosphatidylinositol 3-Kinases / genetics
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Phosphatidylinositol 3-Kinases / metabolism*
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Protein Kinase Inhibitors / pharmacology
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RNA, Messenger / genetics
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Rats
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Transaminases / genetics
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Transaminases / metabolism
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Transcription, Genetic / genetics*
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Wortmannin
Substances
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Androstadienes
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Protein Kinase Inhibitors
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RNA, Messenger
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Transaminases
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acetylornithine transaminase
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Phosphotransferases (Alcohol Group Acceptor)
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inositol polyphosphate multikinase
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Wortmannin