Water Environ Res. 2005 Jul-Aug;77(4):423-4.

False positive fecal coliform in biosolid samples assayed using A-1 medium.

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Environmental Engineering Program, Penn State Capital College, Middletown, PA 17057, USA. khb4@psu.edu

Abstract

Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)--were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.

PMID:: 16121511; [PubMed - indexed for MEDLINE]

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False positive fecal coliform in biosolid samples assayed using A-1 medium.

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