The objective of the present study was to develop a methodology for the quantification of the growth rate of Lemnaceae biomass by digital image analysis. The effect of biomass surface coverage on the oxygen transfer coefficient (Kla) was also quantified. Contribution of Lemnaceae to oxygen balance was evaluated by closed respirometry. Monod-like equations could be derived from growth rate coefficients in various experimental conditions. This opens the way to a deterministic model of Lemnaceae ponds where uptake of nitrogen and phosphorus (even heavy metals) can be calculated.