Source
School of Public Health, Wuhan University, Wuhan 430071, China.
Abstract
OBJECTIVE:
To study on the inhibitory effects of juice of tomato on the growth of human prostate carcinoma PC-3 cells and its possible mechanism.
METHODS:
PC-3 cells were treated with the juice of tomato in different concentration (40, 80, 120 ul/ml) for 48h; the proliferation of PC-3 cells were measured by MTT assay, the comet assay was used to measure the DNA damage of PC-3 cells.
RESULTS:
Juice of tomato could inhibit the proliferation of PC-3 cells, the growth inhibitory rate of experimental groups were significantly higher than that of control group, with very statistical significance; and it could induce the breakage of DNA single strand of PC-3 cells and resulted in comet cells with tail, Rate of DNA tail and the tail length of DNA increased with the increasing of concentration of juice of tomato, showing the obvious dose effect relationship.
CONCLUSION:
Juice of tomato could lead to DNA damage of PC-3 cells, it was related to that could inhibit the proliferation of PC-3 cells.