Mouse anterior pituitary gland: analysis by ion trap mass spectrometry

Neuroendocrinology. 2005;81(4):229-43. doi: 10.1159/000087434. Epub 2005 Aug 10.

Abstract

We investigated the proteome of the anterior pituitary gland (AP) in a species in which the genome has been sequenced. Subcellular fractions of APs from 2-month-old male mice were prepared for protein denaturation, treatment with trypsin and analyses utilizing micro liquid chromatography tandem mass spectrometry and the database search software SEQUEST. In the nuclear, non-nuclear 100,000 g and cytosolic fractions, we identified 49, 36 and 68 different proteins, respectively. A total of 115 distinct proteins were detected. We identified growth hormone, prolactin, pro-opiomelanocortin, the alpha-subunit for the glycoprotein hormones, and luteinizing hormone-beta. Groups of other identified proteins included hormone-processing, secretion granule-associated, non-hormonal endoplasmic reticulum-associated, calcium-binding, protein kinase C-associated, histones, non-histone chromosomal, other RNA-binding, heterogeneous nuclear ribonucleoproteins, splicing factors, helicases, lamins, ribosomal, microtubule-associated, microfilament-associated, adenosine triphosphate- and guanosine triphosphate-associated, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation, enzymes in glycolysis and the tricarboxylic and urea cycles and the pentose phosphate path, heat-shock, glutathione-associated, peroxidases, ubiquitin-associated, catabolic, protease inhibitors, other, and blood proteins. The 115 proteins reported in this study and the 145 proteins reported in a previous study on the AP of the adult male Golden Syrian hamster are compared and form a foundation for defining the proteome in normal adult male AP.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / chemistry
  • Chromatography, Liquid
  • Cricetinae
  • Cytosol / chemistry
  • Male
  • Mass Spectrometry / methods*
  • Mesocricetus
  • Mice
  • Pituitary Gland, Anterior / chemistry*
  • Pituitary Gland, Anterior / ultrastructure
  • Pituitary Hormones / analysis
  • Protein Denaturation
  • Proteome / analysis*
  • Software
  • Trypsin / metabolism

Substances

  • Pituitary Hormones
  • Proteome
  • Trypsin